A Beginners Guide to The Point Spread Function

Suppose that you have a tiny fluorescent object, such as a 10nm-diameter fluorescent bead or even a single fluorescent molecule, and you try to observe it under a fluorescence microscope.  Provided that the object […]

view article

pUC18 – Probably the Best High-Copy plasmid in the World!

Today I would like to introduce you to pUC18, a plasmid most noted for its high copy number. In the first article in this series, we talked about how origins of replication (ori) control plasmid […]

view article

Stop Pushing, Start Enjoying and Get Better Results at the Bench (and in Your Life)

Most of the time, research (and life!) can feel like a struggle. Constant deadlines, incessant demands, pressure to get results, grants, job, publications – and dealing with irritating colleagues and bosses. You know what I […]

view article

Say Goodbye to Restriction Enzymes and Ligases: An Introduction to Sequence and Ligase Independent Cloning (SLIC)

SLIC, or sequence and ligase independent cloning, was developed by Li in 2007 and published in Nature Methods. What makes it a Nature Methods worthy protocol? Unlike other forms of cloning, SLIC does not require restriction enzymes or a ligase! Seriously! Don’t believe […]

view article

Top Ten websites to help you with your PCR experiments

Whether you are embarking on a PhD in molecular biology, trying to amplify a new gene, analyzing gene expression by qPCR, or trying to solve a PCR problem, you will probably resort to a […]

view article

Making The Most Out Of Your Commute To The Lab

Power nap anyone? Depending on how long your commute is, and what type of transport you use, you could make your commute useful. If you are taking public transport, you can use that time […]

view article

The Nature of Denaturing (Protein Gels, that is!)

You've nurtured your cells for weeks, perfected your experimental conditions, and nailed down all the controls. You've harvested your cells and gently lysed them, now you're ready to look at the proteins. What's one […]

view article

The power of STED microscopy, Part 1: How does it work?

Do you suspect that your favourite protein is doing something really cool? But you cannot see it because your confocal microscope’s resolution is limited. Then Stimulated Emission Depletion (STED) microscopy is what you need! […]

view article

Fixation and flow cytometry

Fixation is routinely used in histology and cytology Labs the world over as a way of keeping cells in stasis at a particular point to ensure that, by the time they are examined, they […]

view article

Common Myths of Copyright

Copyright is something that a lot of scientists only give a passing thought to. However, this is something that affects us all. If you publish your work, then you need to understand copyright, the […]

view article
Next Page »

Article Categories